We show that the concentration working range depends on the material’s optical properties, size and concentration. We evaluate the method accuracy, linearity and reproducibility, as well as the operational nanoparticle concentration and size range. We describe the theory of the method and its application to nanoparticles made of gold, silica and polystyrene, with diameters ranging from 30 to 400 nm, and demonstrate some of the limitations with particles of sizes 500 nm and above. The plate scheduler allows the user to graphically set up size and thermal trend measurements of various samples from the same plate using a variety of different SOPs.įor further information about the Zetasizer APS, please visit: present the use of Multi-angle Dynamic Light Scattering (MADLS®) for the measurement of nanoparticle concentration. In addition, thermal trend measurements between 2☌ to 90☌ with 0.1☌ degree precision can be defined. The separate temperature controls of the plate holder and the measurement cell allow the Zetasizer APS to be used to maintain the protein samples in optimal condition until measurement. Setup and operation of the Zetasizer APS is straightforward and cleaning protocols ensure that no cross contamination occurs. Additionally, a new plate navigator feature acts as a data mapping tool, allowing for rapid screening of results to retrieve the information of most interest and the subsequent in-depth investigation of any single data set. The data display capability enables the operator to easily retrieve results of interest, without having to manually scan through all measurements. Zetasizer APS allows the production of large amounts of high quality DLS data with no user intervention to maximize productivity in busy protein laboratories. Easy data interpretation, using custom-designed results criteria, is supported by a colour-coded data quality display. Enabling automated measurements of multiple samples, the Zetasizer APS delivers the same high sensitivity, high specification DLS measurements as other systems in Malvern’s well-known Zetasizer series, making it ideal for protein applications.
12 August 2013: Malvern, UK: A set of application notes from Malvern Instruments demonstrates the sample integrity, flexibility and lack of cross contamination during automated, rapid screening of protein samples using the Zetasizer Auto Plate Sampler (APS) dynamic light scattering (DLS) system.
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